Product Introduction
Papain is a natural proteolytic enzyme obtained from papaya (Carica papaya). It belongs to the cysteine protease family and is capable of hydrolyzing proteins and breaking large protein molecules into smaller peptides and amino acids. Due to its strong proteolytic activity and broad application range, papain is widely used as a food-processing enzyme in meat products, brewing, baking, protein hydrolysates, seafood processing and other food applications. Current food-enzyme assessments also identify papain applications in meat and fish processing, baked products, brewed products, dairy processing and protein hydrolysis.
WEHENGFOOD is a professional Papain manufacturer, supplier and exporter in China, supplying food-grade papain for global food manufacturers, ingredient distributors and industrial users. We can provide different enzyme activity grades according to customers’ formulation and processing requirements, with a commercial activity range of 50,000–2,400,000 U/g. Bulk supply, samples and customized specifications are available for international customers.
Papain works by hydrolyzing peptide bonds in proteins, which makes it particularly useful when manufacturers need to modify protein structure, improve tenderness, enhance protein solubility or produce protein hydrolysates. Its actual performance depends on enzyme activity, dosage, pH, temperature, substrate type and processing time. Published data show that papain’s optimum conditions can vary considerably with the enzyme preparation and assay method, so process parameters should be validated for each application rather than relying on a single universal optimum.

Certificate of Analysis
ITEMS | SPECIFICATIONS | RESULTS |
Appearance | White Powder | Conform |
Odor | Free of offensive odors | Conform |
Mesh | 98% pass 80mesh | Conform |
Activity of enzyme | ≥100,000U/g | 102,700U/g |
Loss on drying | ≤5.0% | 2.20% |
Loss on ignition | ≤5.0% | 3.00% |
Heavy metals | <20mg/kg | <20mg/kg |
Pb | <2.0mg/kg | <2.0mg/kg |
As | <2.0mg/kg | <2.0mg/kg |
Microbiology | ||
Total Plate Count | <10,000cfu/g | 680 cfu/g |
E.Coli | None detected in 10g | Absent |
Salmonella | None detected in 10g | Absent |
Yeast and Mould | NMT 100 cfu/g | conform |
Storage | STORED HUMIDITY PROTECTED |
Shelf Life | 1 Year when Properly stored |
Functions and Usages
Functions of Papain
Papain primarily functions as a proteolytic enzyme. It hydrolyzes proteins into smaller peptides and amino acids, helping food manufacturers modify protein structure and improve processing characteristics. This proteolytic action makes papain especially valuable for meat tenderization, where it can act on myofibrillar proteins and collagen and thereby improve the texture of tougher meat.
In food processing, papain can also be used to produce protein hydrolysates, modify proteins, improve protein solubility and processing performance, and assist in applications such as brewing and baking. Because the required enzyme activity and dosage vary by raw material and process, WEHENGFOOD supplies Papain from 50,000–2,400,000 U/g to support different industrial formulations and processing conditions.
Applications of Papain
Meat Tenderization: Papain is widely used as a meat tenderizing enzyme. By hydrolyzing muscle proteins and connective-tissue proteins such as collagen, it can help improve tenderness and texture in beef, mutton and other processed meat products. Research and food-enzyme assessments support papain’s use in modified meat products and meat protein processing.
Tripe and Meat Processing: Papain can be incorporated into enzyme-based tenderizing formulations for tripe and other relatively tough animal tissues. Controlled enzymatic hydrolysis helps soften protein structures and can reduce dependence on aggressive traditional tenderization treatments. Dosage and treatment time should be optimized according to the raw material and desired texture.
Brewing: Papain can be used during beer and brewed-product processing because of its protein-hydrolyzing properties. By breaking down susceptible proteins and polypeptides, it can assist protein management during processing. Papain is among the food enzymes evaluated for use in brewed products.
Baking: In bakery processing, papain hydrolyzes proteins in flour systems and can modify dough protein structure. Controlled enzymatic treatment can help adjust dough characteristics for specific bakery formulations. Food-enzyme assessments also list baked products among the intended applications of papain.
Protein Hydrolysates: Papain can hydrolyze animal, fish and plant proteins into smaller peptides, making it suitable for the manufacture of protein hydrolysates and protein-based food ingredients. EFSA assessments specifically describe applications involving hydrolysis of meat, fish and plant proteins.
Seafood Processing: Papain can be used for controlled hydrolysis of proteins in fish and shellfish processing. Depending on the process, it can assist with protein modification and the production of hydrolyzed seafood protein ingredients.
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FAQ
Q1: What is Papain?
Papain is a natural proteolytic enzyme derived from papaya. It can break down proteins into smaller peptides and amino acids and is widely used in food processing, meat tenderizing, brewing, baking, and other enzyme applications. WEHENGFOOD is a Papain manufacturer, supplier, and exporter in China, supplying food-grade papain for global customers.
Q2: What is the enzyme activity of your Papain?
WEHENGFOOD supplies Papain with different enzyme activity levels according to application requirements. Available activity can range from 50,000–2,400,000 U/g, and suitable specifications can be selected according to the customer’s formulation and processing needs.
Q3: What are the main applications of Papain in food?
Papain is mainly used for meat tenderization, protein hydrolysis, brewing, baking, and food processing. In meat products, it helps break down muscle proteins and improve tenderness. It can also be used in various protein-processing applications where controlled enzymatic hydrolysis is required.
Q4: What is the minimum order quantity for Papain?
Our standard MOQ for Papain is 100 KGS. For distributors, food manufacturers, and industrial users requiring larger quantities, WEHENGFOOD can provide competitive bulk pricing and stable supply from China.
Q5: What packaging do you provide for Papain?
Our standard Papain packaging is 25 kg/carton. Export packaging is designed for bulk transportation and international shipments. Please contact us if you have specific packaging or labeling requirements.
Q6: Why choose WEHENGFOOD as your Papain supplier in China?
WEHENGFOOD is a professional Papain manufacturer, supplier, and exporter in China, providing food-grade Papain with enzyme activity options of 50,000–2,400,000 U/g. We support bulk supply, specification selection, technical documentation, samples, and international export services for food manufacturers, distributors, and ingredient importers worldwide.











